Vitamin Assay Methods

Specificity and relative efficacy of different assay methods for thiamine hydrochloride in vitamin B-complex syrups.

Vitamin B-complex syrups were prepared using locally manufactured sucrose and liquid glucose vehicles, and stored at room temperature, 37°C and 45°C, for 6-12 months to evaluate the stability of thiamine hydrochloride. The stability testing of the syrups has been designed to determine quantitatively and qualitatively the changes which they undergo during storage. The chemical stability has been...

Immunonephelometric assay of vitamin D-binding protein.

An automated immunonephelometric assay was developed to measure vitamin D-binding protein (DBP) in serum. The assay is described for the Behring Nephelometer System, which uses rabbit anti-DBP antiserum and purified human DBP. The detection limit is 0.05 g/L (0.86 mumol/L), and the working range is less than or equal to 1.60 g/L (27.59 mumol/L). Intra- and interassay CVs of 2.0% and 2.8-3.8% co...

Clinical application of serum vitamin B12 assay.

Interlaboratory comparison of serum vitamin B12 assay.

The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in...

Methods to assay Drosophila behavior.

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases(1). We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely ap...